Analysis of common problems in cell culture

Analysis of common problems in cells

1. How should the freezing tube be thawed?

After removing the cryotube, it should be quickly thawed in a 37 °C water tank. Gently shake the cryotube to melt it in 1 minute. Note that the water surface should not exceed the edge of the frozen tube, otherwise it will be prone to contamination. In addition, when the frozen tube is taken out from the liquid ammonia barrel, it must be safe to prevent the freezing tube from bursting.

2. Can I use a medium different from the original culture conditions?

No. Each cell strain has its own specific cell culture medium. If the culture medium with different culture conditions is used suddenly, the cells are mostly unable to adapt immediately, and the cells cannot survive.

3. Can I use serum types different from the original culture conditions?

No. Serum is an extremely important source of nutrients in cell culture, so the type and quality of serum can have a significant impact on cell growth. Serum from different specialties varies in the amount or content of some substances or molecules, and the use of serum often causes the cells to fail to survive.

4. How should the suspended cells be treated in a subculture?

Generally, it is only necessary to continuously add fresh medium to the original culture flask to dilute the cell concentration. If the culture solution is too much, the mouth of the culture flask can be raised slightly until it cannot be accommodated. When the bottle is dispensed, take out a part of the culture medium containing the cells to another new culture flask, and add the fresh medium to the appropriate concentration, and repeat the above steps.

5. What kind of speed should the centrifugal rate be to centrifuge the general animal cells?

Animal cells to be recovered, which was centrifuged at 300xg rate is generally (about 1, 000rpm), 5-10 minutes, the speed is too high, will cause cell death.

6. What should I do if the cells are slightly contaminated?

Add the appropriate antibiotics. Discard after direct sterilization.

7. Is the dish and flash used in various cell cultures the same?

Different brands of dish or flash, they are different coating of polymer, the manufacturing process is also different, although not much impact on the majority of the cells, but a few may be due to the use of different brands of dish or flask while there are notable differences in growth .