Thermo Fisher Scientific offers solutions for plasticizers in food

Thermo Fisher Scientific offers solutions for plasticizers in food

Taiwan's plasticizers have become more and more intense like snowballs, which has caused a major food safety crisis. The food that was eaten and drunk was "all-in-one", and the people smelled "plastic". At present, at least 156 companies in Taiwan have been affected by plasticizers, and the number of contaminated products has expanded to nearly 500...
The "plasticizer" herein refers to a plastic softener for industrial use. There are two main types involved in this food storm: diethylhexyl phthalate (DEHP), which is commonly used in seats, car sofas, etc.; diisodecyl phthalate (DINP), commonly used in children's toys. . Others such as dibutyl phthalate (DBP) and butyl butyl phthalate (BBP) are widely used plasticizers.
"Plasticizer" DEHP, DINP act like artificial hormones, long-term accumulation of high doses in the body, may cause children's gender disorder, including genital shortening, sexual characteristics are not obvious, although it is not confirmed whether it is carcinogenic to humans, but animals will cause cancer reaction. Plasticizers may not be added to foods according to law.
This article mainly provides several common "plasticizers" detection methods.
First, the sample pretreatment method
1. Solid phase extraction column: HyperSep C8 (3ml/200mg, catalog number 60108-393);
2. Activation: 5 mL of dichloromethane, 5 mL of methanol, 5 mL of purified water;
3. Loading: 20mL beverage sample, depressurized column, flow rate <5ml / min; after loading, drain SPE column for 5min;
4. Washing: 3mL 5% aqueous methanol solution;
5. Elution: 1 mL Acetone: Ethyl acetate (3:1) eluted, concentrated and injected. Reversed-phase chromatography was carried out after drying the eluate and re-sizing with methanol.
Second, HPLC & GC method
HPLC method (reverse phase)
1, high performance liquid chromatography, with autosampler or manual injector, a multi-wavelength detector (detection wavelength of 225nm) or a DAD detector (200 ~ 400nm wavelength detection whole).
2. Column: Hypersil BDS C18 column (250mm × 4.6mm, particle size 5μm, pore size 130A, carbon loading 11%, 28105-254630)
a. Column temperature: 35 ° C
b. Mobile phase: water (A)-methanol (B)
c Gradient:. 15% A (0 ~ 8min) à 0% A (8 ~ 10min) à 0% A (10 ~ 20min)
d. Flow rate: 1ml/min
e. Injection volume: 20μL
3. The chromatogram is as follows:
HPLC method (normal phase)
1. High performance liquid chromatograph with autosampler or manual injector, multi-wavelength detector (detection wavelength 225nm) or DAD detector (200~400nm full wavelength detection).
b. Mobile phase: n-octane
c. Flow rate: 0.5ml/min
d. Injection volume: 20μL
3. Under the above conditions, the retention time of DINP (Peak 2) is about 3.2min.
GC method
1, GC should be equipped on-column injector, or PTV injector, or splitless injector. With FID detector.
2, column and chromatographic conditions
a. Column: TR-5MS 30mm x 0.25mm x 0.25 (26098-1420);
b. Sampler: splitless injection, 1μl;
c. 18 ° C / min from 120 ° C to 300 ° C;
d. inlet temperature: 280 ° C;
e. Carrier gas: helium, 1.5ml/min;
f. Detection: FID 320 °C.

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