The heat is approaching, it is the high incidence of fever. In order to understand the resistance of pig herds to blue ear disease and circumcision, there are many farms that regularly perform antigen and antibody tests. However, farmers found that submitting the same blood sample to different laboratories sometimes resulted in completely different results and made it difficult to understand. Fan Fuhao, Director of the Genetic Analysis Center of the Ministry of Agriculture's Breeding Quality Inspection Center (hereinafter abbreviated as the Measurement Center), believes that the above situation will occur in blood sample contamination or laboratory operation errors. Among them, corruption or hemolysis and lipemia are the most common blood samples during transportation. Case. Therefore, it is very important for the pigs to do antigen and antibody tests for the correct preservation of blood samples. In addition, farmers can also choose to do health index assessments. Farmers favor antibody screening In early April, Chen Guangrun, head of Liantang Pig Farm in Jiangmen City, Guangdong Province, which had 380 fertile sows, collected some blood samples from pigs of different stages of growth and sent them to the Veterinary Research Institute of Guangdong Academy of Agricultural Sciences for routine antibody against swine diseases. Testing. According to Chen Guangrun, when the season changes or the epidemic is unstable, it has become a routine practice for pig farms to do antibody testing to assess the health of the herd. According to CAI Jianjian, a pig ward doctor from the Institute of Veterinary Medicine of the Guangdong Academy of Agricultural Sciences, in recent years, large- and medium-scale farms (more than 100 sows per year) have paid more and more attention to the regular census of herds of antibodies, mainly for swine fever, The detection of pseudorabies and blue-eared antibodies is about twice a year, and the pig farms are usually tested when the outbreak is unstable or there is a problem. Huang Jiaren of Lion Farm in Shihai, Shihai, South China Sea believes that large pig farms will have higher quality of talents than small pig farms, and have a high degree of acceptance of detection technologies. Therefore, pig farm antibody screening will do more; and pig farms will have funds and technical strength. Limited, analysis of test results is not clear, not transparent, meaning little for production guidance, so the recognition of antibody screening is not high. Mai Zhi, deputy general manager of New Food Group Co., Ltd., said that the annual cost of the company's censoring of antibodies is huge, mainly based on monitoring breeding herds, and about 15% of the samples are sampled. Mai Zhi revealed that the company mainly sent blood samples to the testing center or the Veterinary Institute and Da Huanong emerging R&D center. It is understood that the detection rate of swine fever antibody is around RMB 10/serving, that of PRV antibody is RMB 20/unit, and that of blue-eared antibody is RMB 50/unit, while the detection of virus is between RMB 50 and RMB 100 each. In recent years, due to the popularity of antibody screening technology, many businesses have also taken a look at the market in this area. Many veterinary drug distribution companies also started to launch this project. For example, Heyuan Kanghejian Animal Health Products Co., Ltd. established a pig disease laboratory in 2008 to provide customers with antibody testing services. In the kit market, in 2010, following the introduction of the ring vaccine by major manufacturers, Tianjin Ripu Biotechnology Co., Ltd. was the first to launch a ring antibody test kit in China to compete in the diagnostic reagent market. Blood sample contamination can lead to detection failure Although antibody detection technology has continued to mature, it often happens that the detection fails. "The same blood sample was sent to different laboratories and the results were sometimes completely different." Many farmers reported: "I don't know which one to believe." Fan Fuhao analyzes that blood sample pollution or laboratory operation errors will lead to failure of monitoring. Among them, corruption or hemolysis and lipemia are the most common conditions in blood samples during transportation. "Of course, the equipment of each laboratory is different, and its sensitivity will be different." Fan Fu believes that as long as the operation is correct, there will not be much deviation from the monitoring results of the pigs. As for the preservation of blood samples, it is a big problem at present. Fan Fuhao introduced that the center has developed a blood protection solution that uses non-chemical preservatives to make blood samples less prone to contamination and spoilage and prolong blood storage. When the blood is collected, it is only necessary to extract the same volume of protective liquid and mix it evenly, and then send it to the laboratory together with the syringe. Fan Fuhao introduced that in contrast tests, the results of the use of blood protection samples, both antigen detection and antibody detection, were accurate. "Current blood protection fluid is provided free of charge to the inspection site." Fan Fu said, and because of the 1:1 addition of blood protection solution, the blood sample was diluted in advance, so the blood sample dilution step or dilution factor in the ELISA experiment can be reduced. Saves reagent costs. Fan Fu revealed that they are planning to apply for a patent for blood protection solution. Health Index Evaluation Focused Fan Fu believes that, in addition to the above-mentioned factors affecting the test results, in the increasingly complex domestic breeding environment, the blind pursuit of the detection and monitoring of antigens and antibodies has reduced its guiding significance because it is impossible to find the most needed primary origins. Sexual pathogens. Fang Shuhe, technical service manager of Boehringer Ingelheim Trading Co., Ltd. in South China, also believes that the domestic swine herd infection is under greater pressure and many pigs are in a sub-healthy state; viruses and antibodies are in a dynamic equilibrium. Therefore, the detection of a virus in a pig body can only indicate that the body is poisonous, but it cannot be said that the disease is present in the field. "So we recommend to do a health index assessment for the pigs." Fan Fu said that the health index assessment mainly includes five aspects: nutrition, poisoning, immunosuppression, inflammation and septicemia, also referred to as "two and a half" tests. "It is a wrong thinking to think about the cause of a virus or bacteria blindly after a problem. Often, the problem lies in external and objective factors such as nutrition or management." Fan Fu thinks that sometimes the disease is a scapegoat. As far as the current round of vaccine antibody monitoring concerns for farmers, Fan Fu thinks that the ring antibody detection kit is not very mature and has poor stability and repeatability. If you want to assess the effectiveness of the vaccine, it is best to do a comparison experiment. "I used to do comparative experiments before, one group of immunizations imported a ring vaccine, and one group was not immune; the difference after 40 days is very significant." Cai Yujian also believes that the level of detection of antibody levels in the ring is not significant, "mainly to see The antibody is uniform and uniform.â€
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Diagnosis of swine disease from clinical anatomy to laboratory testing