Preparation of Edible Fungi Liquid Bacteria

After installing the ventilator, oxygen is supplied to the medium using an oxygen feeder.

The culture medium was shaken on a shaker.

Edible fungus liquid strains have the advantages of short production cycle, uniform and consistent bacterial age, convenient inoculation, fast fermentation, and suitable for industrial production. The production methods of edible mushroom liquid strains can generally be divided into electromagnetic stirring method, shaker bottle method, simple deep fermentation (blowing oxygen) method, and fermentation tank method. According to the staff of the Yunnan Academy of Agricultural Sciences, the bottle shaker method is not only simple to inoculate, but also easy to manage, and the amount of production varies depending on the settings of the machine. You can invest 2000 to 3,000 yuan.

Prepare the liquid culture medium. First, cook the potatoes until they are cooked, but when they are not rotten, extract the supernatant. Then prepare 3% glucose, 1% corn flour, 0.05% magnesium sulfate, 0.1% potassium dihydrogen phosphate, and finally the potato supernatant and the above Solution and water can be mixed. The liquid medium is suitable for the cultivation of a variety of edible mushroom species.

Shake flask shake liquid culture medium is prepared, then put into a 500 ml Erlenmeyer flask, each bottle is filled with 100 ml, and 1-15 small glass beads are added, and the bottle mouth is sealed with tampon and kraft paper at 1.5 kg. Sterilize at 30 cm/cm2 for 30 minutes. Then, a piece of slanted bacterium of about 2 cm2 was placed and incubated at 23°C to 25°C for 24 hours. When the mycelium is germinated, it is placed on a reciprocating shaker and cultured at an oscillation frequency of 80 to 100 beats per minute with an amplitude of 6 to 10 cm. The temperature of the shaker room is controlled at 24°C to 25°C, and the culture time varies with the fungus, usually about 5 days. The oscillating medium is poured into a large container. After expansion, the aeration tube is installed under aseptic conditions, and oxygen is passed through the oxygen feeder to incubate an appropriate amount of oxygen for 48 hours. The standard of culture completion: The culture solution is clear and transparent, in which a large number of small mycelial balls are suspended, accompanied by the unique flavors of various mushrooms. Cultured liquid bacteria should be kept in the incubator.

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