Fluorescent western Blot: Choose visible fluorescence detection or infrared detection? One of the main advantages of fluorescence detection is the ability to perform multiple assays (two or more proteins). Current experimental techniques allow the detection of two proteins using near-infrared detection and the detection of three proteins using three-color RGB visible fluorescence. Advantages of near infrared detection Simultaneous detection of three proteins is better than two. Why do we still choose infrared imaging? Infrared fluorescence detection of target proteins on blotting membranes is an effective western blot method, especially in detecting weak signals of low-abundance proteins. Near-infrared detection is the best choice. The NC (nitrocellulose) membrane is autofluorescent, and the background noise in the infrared region is much lower than that in the visible fluorescence region, and the signal-to-noise ratio is higher, making it easier to detect the target protein. Laser-infrared western The Azure c-Series imaging system is excited by a laser source that emits light closer to the maximum emission wavelength of the dye at 680 nm and 800 nm, and the secondary antibody produces a higher signal. Visible fluorescence detection advantages When three proteins need to be detected simultaneously, it is apparent that fluorescence is the best choice. Visible fluorescence is very flexible and there are a variety of visible fluorescent dyes to choose from. If you are using other visible fluorescent dyes (the emission spectra can be distinguished) for other experiments, it is easy to apply these dyes to Western blot experiments. Shanghai Jiecheng Biotechnology Co., Ltd. http://
Human chorionic gonadotropin is a hormone for the maternal recognition of pregnancy produced by trophoblast cells
Human chorionic gonadotropin (HCG) is a glycoprotein secreted by trophoblast cells of the placenta And β dimer proteins. The molecular weight of glycoprotein hormone 36700, A. Pituitary, FSH, follicle stimulating hormone, LH (luteinizing hormone) and TSH (thyroid stimulating hormone) are basically similar, So they can cross-react with each other, while the structure of β subunit is not similar. In mature women, the fertilized ova move to the uterine cavity for implantation and form embryos. In the process of development and growth into the fetus, the placental syncytiotrophoblast cells produce a large amount of HCG, which can be excreted into urine through the blood circulation of pregnant women. Serum and urine HCG levels rise Rapidly from 1 to 2.5 weeks of gestation, reaching a peak at 8 weeks of gestation and dropping to moderate levels at 4 months of gestation, which remain at the end of gestation. At present, the commonly used detection methods are: latex aggregation inhibition test and hemagglutination inhibition test; Radioimmunoassay (RIA); Enzyme linked immunosorbent assay (ELISA); Monoclonal antibody colloidal gold test
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Fluorescent western Blot: Choose visible fluorescence detection or infrared detection?