Nursing Care of Frozen Herbs

Since mid-January of this year, many provinces in China have suffered from prolonged freezing weather, which has led to severe freezing of woody medicinal herbs. In order to reduce losses, it is necessary to strengthen the following management in a timely manner.

1. Trimming frozen leaves is a kind of self-protection measures to resist freezing injury of the woody medicinal tree. Through defoliation, the abnormal loss of water can be reduced and the tree body can be prevented from dying due to excessive water loss. Post-frozen defoliation will delay the germination. Therefore, when the branches sprout and after the dead branches can be distinguished, the dead branches, the diseased shoots, and the survival branches and the remaining leaves must be promptly removed. It is difficult to distinguish between the live and dead branches. Trimming. The pruning action should be light, the clipping should be smooth, and the large cut point should be disinfected with 75% alcohol solution or 0.1% potassium permanganate solution and wrapped with a thin film.

Second, fertilization after freezing woody medicinal tree generally weakened, frostbite tree root water absorption, poor suction capacity. Therefore, after the woody medicinal herbs are frozen, the roots should be top-dressed, and fertilization should be applied lightly, thinly, and repeatedly to promote strong new shoots and restore vigor. Nitrogen, phosphorus, and potassium-based quick-acting fertilizers are the main fertilizers. It is advisable to use foliar spray fertilizers after the occurrence of new shoots.

Third, the prevention of pests and freezes is likely to cause damage to branches, leaves, resulting in exposed branches, the future is very prone to serious pests and diseases, such as resin disease, sunburn cracking disease. Therefore, it is necessary to promptly prevent diseases and treat diseases after freezing. The prevention and treatment of diseases can use Bordeaux mixture, carbendazim and other agents, and can prevent and treat insect pests such as dichlorvos and trichlorfon.

The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971.[1] The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.

In the most simple form of an ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change.

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