Our organic vegetable powder mainly used dehydration technology. Dehydration vegetable powder remained most fiber and taste of fresh vegetables, so they are suitable to be added in sauce, stuff, pudding, yogurt and dessert. Some vegetable powder could be applied as pigment like red beet root powder. Some could be mixed with other super green powders to make health formula, kale powder and broccoli powder are always good choice for customers.
Vegetable Powder,Red Beet Root Powder,Broccoli Powder,Organic Vegetable Powder YT(Xi'an) Biochem Co., Ltd. , https://www.ytherblifes.com
RNase contamination prevention measures
The chemical nature of RNA is much more active than DNA. The 2' hydroxyl group immediately adjacent to the phosphodiester bond on the RNA molecule can be directly utilized by RNase as an active factor, so that RNases can be activated without metal ions. Because RNase is widely present in the environment, especially RNase A, the structure is extremely stable and cannot be inactivated by autoclaving, which is extremely difficult to eliminate and poses a great threat to the integrity of RNA samples. Since the RNase class A enzyme is catalyzed by a histidine residue at the active site, it can be inhibited by the histidine alkylating agent DEPC. In order to avoid RNA degradation, RNA-related experiments are strictly following the following rules of operation:
1. Use RNase-free solution;
2. Wear clean disposable gloves and replace them frequently;
3. Establish an RNA work area, using a dedicated pipette and RNase-free tips;
4. Use sterile disposable plastic utensils to avoid the use of glassware;
5. Metal tools can quickly eliminate pollution by burning for a few seconds;
6. The glassware can be baked at a temperature above 180 ° C for several hours, or after soaking for 1 hour with freshly prepared 0.1% (v / v) DEPC or absolute ethanol, autoclaving to remove residual DEPC;
7. Containers containing polycarbonate or polystyrene in electrophoresis tanks should be immersed in 3% hydrogen peroxide for 10 minutes, then rinsed with plenty of RNase-free water for use;
8. Plastic products can be soaked in freshly prepared 0.1% (v/v) DEPC water overnight, then autoclaved for 1 hour to hydrolyze residual DEPC;
9. Reagent treatment:
1) Add 1 ml of DEPC per liter of solution, shake at room temperature overnight or shake at 37 ° C for 1 hour, then autoclave for 1 hour to hydrolyze residual DEPC;
2) Since DEPC can react with the primary amino group, the solution containing Tris should not be treated with DEPC. Should use a new open bottle (RNA-specific) Tris powder, dissolved in DEPC treated water or Milli-Q water, apply RNA-specific electrodes to adjust the pH value, then autoclave;
3) For compounds that are unstable to heat (such as DTT, nucleosides, manganese salts, etc.), they should be directly dissolved in DEPC treated water or Milli-Q water, and sterilized by filtration using a 0.2 μm filter;
4) Reagents such as anhydrous ethanol and isopropanol are used for RNA after opening; 75% ethanol should be prepared by using anhydrous ethanol and DEPC treated water;
5) An RNase inhibitor is added to the reaction system.
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