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China Microscope - Wang Liang: 010-82967128 13910386343 Optical microscope use precautions
**How to Use an Optical Microscope**
Using an optical microscope requires careful handling and proper technique to ensure clear and accurate observations. Here’s a step-by-step guide to help you operate the microscope effectively.
First, hold the microscope correctly. The right hand should support the mirror arm, while the left hand holds the base. Place the microscope on your chest or desk for stability. When rotating the microscope, make sure the lens barrel is facing forward and the mirror arm is at the back. Position it in front of you, slightly leaning to the left so that your left eye can easily look through the eyepiece. Keep the microscope about 5 cm away from the edge of the table for better control.
For adjusting the light, start by slowly raising the lens barrel using the coarse focus knob. Then rotate the nosepiece to align the low-power objective with the light hole. Adjust the diaphragm (or aperture) with your finger to open it as wide as possible. While looking through the eyepiece with your left eye, turn the mirror to face the light source until the field of view is evenly illuminated.
When using the low-power objective, gently lower the lens tube with the coarse focus knob while watching from the side with both eyes. Stop when the objective is about 2–3 mm above the slide. Then, use your left eye to look through the eyepiece and slowly raise the lens tube until the object comes into view. If the image is blurry, adjust the fine focus knob until it becomes sharp.
Before switching to a high-power objective, locate the object under low power and center it in the field of view. Rotate the nosepiece to change to the high-power lens. The field of view may become darker, so increase the aperture size or use the concave mirror for better illumination. Then, adjust the fine focus knob for clarity. Objects will appear larger but fewer in number.
Mirrors are used with the diaphragm to control brightness. A flat mirror is used for strong light, while a concave mirror is used for dimmer conditions. If the light is too bright, reduce the aperture size or switch to a flat mirror. If it's too dark, use a larger aperture or the concave mirror.
To clean the lenses, always use special lens paper. Fold it several times and wipe in one direction only—never back and forth. If the lens is oily, apply a few drops of xylene onto the paper and wipe gently as described.
Remember, the magnification of the microscope refers to the length and width of the object, not its area or volume. The focal length is the distance between the objective lens and the specimen. When moving the slide, the object appears to move in the opposite direction.
To check for foreign particles, move the slide to see if the particle moves (indicating it's on the slide), or rotate the nosepiece to see if it remains (indicating it's on the objective). If it stays in the eyepiece, it’s likely on the eyepiece itself.
After finishing your experiment, remove the slide, wipe the mechanical parts with a clean cloth, and rotate the nosepiece so that the objectives are positioned on either side. Lower the lens tube to its lowest point, stand the mirror upright, cover the microscope with a red silk cover, and lock it back in the case for safe storage.
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